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uc berkeley rrid addgene 29656 pet28a expression vector sigma aldrich  (Addgene inc)


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    Structured Review

    Addgene inc uc berkeley rrid addgene 29656 pet28a expression vector sigma aldrich
    Uc Berkeley Rrid Addgene 29656 Pet28a Expression Vector Sigma Aldrich, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28a+lic+expression+vector/pET+His6+MBP+TEV+LIC+cloning+vector+(1M)+(Plasmid+%2329656)/10__1016_slash_j__isci__2025__112534-686-16-19
    Average 94 stars, based on 98 article reviews
    uc berkeley rrid addgene 29656 pet28a expression vector sigma aldrich - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Purification:

    Article Title: Kinetic Mechanism of Protein N-terminal Methyltransferase 1
    Article Snippet: .. Purification of NTMT1—His-NTMT1 was expressed in Escherichia coli BL21 (DE3) codon plus RIL cells in Terrific Broth medium in the presence of 50 g/ml kanamycin, using a pET28a-LIC expression vector that encodes a full-length NTMT1 (amino acids 1–222) with His6 tag obtained from Addgene. ..

    Article Title: Kinetic Mechanism of Protein N-terminal Methyltransferase 1
    Article Snippet: The AdoHcy hydrolase (SAHH) clone was obtained through a Materials Transfer Agreement with Dr. Raymond C. Trievel (University of Michigan) and was expressed and purified as described by Collazo et al. ( 13 ). . Peptides representing hRCC1-6 (SPKRIA), hRCC1-9 (SPKRIAKRR), hRCC1-10 (SPKRIAKRRS), and hRCC1-12 (SPKRIAKRRSPP) were synthesized on Rink amide resin using standard Fmoc chemistry with a CEM Liberty microwave peptide synthesizer. .. Purification of NTMT1 His-NTMT1 was expressed in Escherichia coli BL21 (DE3) codon plus RIL cells in Terrific Broth medium in the presence of 50 μg/ml kanamycin, using a pET28a-LIC expression vector that encodes a full-length NTMT1 (amino acids 1–222) with His 6 tag obtained from Addgene. ..

    Expressing:

    Article Title: Kinetic Mechanism of Protein N-terminal Methyltransferase 1
    Article Snippet: .. Purification of NTMT1—His-NTMT1 was expressed in Escherichia coli BL21 (DE3) codon plus RIL cells in Terrific Broth medium in the presence of 50 g/ml kanamycin, using a pET28a-LIC expression vector that encodes a full-length NTMT1 (amino acids 1–222) with His6 tag obtained from Addgene. ..

    Article Title: Development of Novel Small-Molecule Activators of Pyruvate Kinase Muscle Isozyme 2, PKM2, to Reduce Photoreceptor Apoptosis
    Article Snippet: .. A pET28a-LIC expression vector containing PKM2 (residues 1–531) with an N-terminal, non-cleavable His 6 tag (MGSSHHHHHHSSGLVPRGS) was purchased from Addgene (Watertown, MA, USA, Addgene plasmid # 25360). ..

    Article Title: Kinetic Mechanism of Protein N-terminal Methyltransferase 1
    Article Snippet: .. His-NTMT1 was expressed in Escherichia coli BL21 (DE3) codon plus RIL cells in Terrific Broth medium in the presence of 50 μg/ml kanamycin, using a pET28a-LIC expression vector that encodes a full-length NTMT1 (amino acids 1–222) with His 6 tag obtained from Addgene. ..

    Article Title: Kinetic Mechanism of Protein N-terminal Methyltransferase 1
    Article Snippet: The AdoHcy hydrolase (SAHH) clone was obtained through a Materials Transfer Agreement with Dr. Raymond C. Trievel (University of Michigan) and was expressed and purified as described by Collazo et al. ( 13 ). . Peptides representing hRCC1-6 (SPKRIA), hRCC1-9 (SPKRIAKRR), hRCC1-10 (SPKRIAKRRS), and hRCC1-12 (SPKRIAKRRSPP) were synthesized on Rink amide resin using standard Fmoc chemistry with a CEM Liberty microwave peptide synthesizer. .. Purification of NTMT1 His-NTMT1 was expressed in Escherichia coli BL21 (DE3) codon plus RIL cells in Terrific Broth medium in the presence of 50 μg/ml kanamycin, using a pET28a-LIC expression vector that encodes a full-length NTMT1 (amino acids 1–222) with His 6 tag obtained from Addgene. ..

    Article Title: The TINCR ubiquitin-like microprotein is a tumor suppressor in squamous cell carcinoma
    Article Snippet: To detect mutations in Trp53 from UVB-induced mouse lesions, we performed dideoxynucleotide DNA sequencing of PCR products encompassing the Trp53 coding exons in DNA-extracted formalin-fixed paraffin-embedded mouse papilloma and squamous cell carcinoma samples using standard procedures at Genewiz (South Plainfield, New Jersey). .. We amplified the human TINCR coding sequence by PCR from pcDNA 3.1-TESO and cloned it in-frame following an N-terminal hexahistidine (His 6 ) tag in BseR I linearized pET28a-LIC expression vector (Addgene 26094). ..

    Plasmid Preparation:

    Article Title: Development of Novel Small-Molecule Activators of Pyruvate Kinase Muscle Isozyme 2, PKM2, to Reduce Photoreceptor Apoptosis
    Article Snippet: .. A pET28a-LIC expression vector containing PKM2 (residues 1–531) with an N-terminal, non-cleavable His 6 tag (MGSSHHHHHHSSGLVPRGS) was purchased from Addgene (Watertown, MA, USA, Addgene plasmid # 25360). ..

    Article Title: Kinetic Mechanism of Protein N-terminal Methyltransferase 1
    Article Snippet: .. His-NTMT1 was expressed in Escherichia coli BL21 (DE3) codon plus RIL cells in Terrific Broth medium in the presence of 50 μg/ml kanamycin, using a pET28a-LIC expression vector that encodes a full-length NTMT1 (amino acids 1–222) with His 6 tag obtained from Addgene. ..

    Article Title: Kinetic Mechanism of Protein N-terminal Methyltransferase 1
    Article Snippet: The AdoHcy hydrolase (SAHH) clone was obtained through a Materials Transfer Agreement with Dr. Raymond C. Trievel (University of Michigan) and was expressed and purified as described by Collazo et al. ( 13 ). . Peptides representing hRCC1-6 (SPKRIA), hRCC1-9 (SPKRIAKRR), hRCC1-10 (SPKRIAKRRS), and hRCC1-12 (SPKRIAKRRSPP) were synthesized on Rink amide resin using standard Fmoc chemistry with a CEM Liberty microwave peptide synthesizer. .. Purification of NTMT1 His-NTMT1 was expressed in Escherichia coli BL21 (DE3) codon plus RIL cells in Terrific Broth medium in the presence of 50 μg/ml kanamycin, using a pET28a-LIC expression vector that encodes a full-length NTMT1 (amino acids 1–222) with His 6 tag obtained from Addgene. ..

    Article Title: The TINCR ubiquitin-like microprotein is a tumor suppressor in squamous cell carcinoma
    Article Snippet: To detect mutations in Trp53 from UVB-induced mouse lesions, we performed dideoxynucleotide DNA sequencing of PCR products encompassing the Trp53 coding exons in DNA-extracted formalin-fixed paraffin-embedded mouse papilloma and squamous cell carcinoma samples using standard procedures at Genewiz (South Plainfield, New Jersey). .. We amplified the human TINCR coding sequence by PCR from pcDNA 3.1-TESO and cloned it in-frame following an N-terminal hexahistidine (His 6 ) tag in BseR I linearized pET28a-LIC expression vector (Addgene 26094). ..

    Amplification:

    Article Title: The TINCR ubiquitin-like microprotein is a tumor suppressor in squamous cell carcinoma
    Article Snippet: To detect mutations in Trp53 from UVB-induced mouse lesions, we performed dideoxynucleotide DNA sequencing of PCR products encompassing the Trp53 coding exons in DNA-extracted formalin-fixed paraffin-embedded mouse papilloma and squamous cell carcinoma samples using standard procedures at Genewiz (South Plainfield, New Jersey). .. We amplified the human TINCR coding sequence by PCR from pcDNA 3.1-TESO and cloned it in-frame following an N-terminal hexahistidine (His 6 ) tag in BseR I linearized pET28a-LIC expression vector (Addgene 26094). ..

    Sequencing:

    Article Title: The TINCR ubiquitin-like microprotein is a tumor suppressor in squamous cell carcinoma
    Article Snippet: To detect mutations in Trp53 from UVB-induced mouse lesions, we performed dideoxynucleotide DNA sequencing of PCR products encompassing the Trp53 coding exons in DNA-extracted formalin-fixed paraffin-embedded mouse papilloma and squamous cell carcinoma samples using standard procedures at Genewiz (South Plainfield, New Jersey). .. We amplified the human TINCR coding sequence by PCR from pcDNA 3.1-TESO and cloned it in-frame following an N-terminal hexahistidine (His 6 ) tag in BseR I linearized pET28a-LIC expression vector (Addgene 26094). ..

    Polymerase Chain Reaction:

    Article Title: The TINCR ubiquitin-like microprotein is a tumor suppressor in squamous cell carcinoma
    Article Snippet: To detect mutations in Trp53 from UVB-induced mouse lesions, we performed dideoxynucleotide DNA sequencing of PCR products encompassing the Trp53 coding exons in DNA-extracted formalin-fixed paraffin-embedded mouse papilloma and squamous cell carcinoma samples using standard procedures at Genewiz (South Plainfield, New Jersey). .. We amplified the human TINCR coding sequence by PCR from pcDNA 3.1-TESO and cloned it in-frame following an N-terminal hexahistidine (His 6 ) tag in BseR I linearized pET28a-LIC expression vector (Addgene 26094). ..

    Clone Assay:

    Article Title: The TINCR ubiquitin-like microprotein is a tumor suppressor in squamous cell carcinoma
    Article Snippet: To detect mutations in Trp53 from UVB-induced mouse lesions, we performed dideoxynucleotide DNA sequencing of PCR products encompassing the Trp53 coding exons in DNA-extracted formalin-fixed paraffin-embedded mouse papilloma and squamous cell carcinoma samples using standard procedures at Genewiz (South Plainfield, New Jersey). .. We amplified the human TINCR coding sequence by PCR from pcDNA 3.1-TESO and cloned it in-frame following an N-terminal hexahistidine (His 6 ) tag in BseR I linearized pET28a-LIC expression vector (Addgene 26094). ..

    other:

    Article Title: Kinetic Mechanism of Protein N-terminal Methyltransferase 1
    Article Snippet: Nickel-nitrilotriacetic acid resin was used as purchased from Qiagen.



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